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Goat anti-ATF2

EB05198
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetATF2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ATF2 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1386
  • Target name
    ATF2
  • Target description
    activating transcription factor 2
  • Target synonyms
    CRE-BP1, CREB-2, CREB2, HB16, TREB7, cyclic AMP-dependent transcription factor ATF-2, cAMP response element-binding protein CRE-BP1, cAMP responsive element binding protein 2, formerly, cAMP-dependent transcription factor ATF-2, cyclic AMP-responsive element-binding protein 2, histone acetyltransferase ATF2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001871.2. GeneIDs all Nonhuman: 81647 (rat);. Purification: Antigen affinity purified. Names and symbols: ATF2; activating transcription factor 2; HB16; CREB2; TREB7; CRE-BP1; cAMP responsive element binding protein 2; MGC111558; activating transcription factor 2 splice variant ATF2-var2; cAMP responsive element binding protein 2, formerly
  • Reactivity Supplier
    Human, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATF2 using anti-ATF2 antibody (A00916-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF2 antigen affinity purified polyclonal antibody (Catalog # A00916-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF2 at approximately 75 kDa. The expected band size for ATF2 is at 55 kDa.
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