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Wild-type (WT) and ATG14 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX119950) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Wild-type (WT) and ATG14 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX119950) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Wild-type (WT) and ATG14 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG14 antibody (GTX119950) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

ATG14 antibody

GTX119950
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetATG14
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Overview

  • Supplier
    GeneTex
  • Product Name
    ATG14 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID22863
  • Target name
    ATG14
  • Target description
    autophagy related 14
  • Target synonyms
    ATG14L, BARKOR, KIAA0831, beclin 1-associated autophagy-related key regulator, ATG14 autophagy related 14 homolog, Beclin 1-Interacting protein, autophagy-related protein 14-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZNE5
  • Protein Name
    Beclin 1-associated autophagy-related key regulator
  • Scientific Description
    Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Lee J, Kim J, Shin J, et al. ATG101 Degradation by HUWE1-Mediated Ubiquitination Impairs Autophagy and Reduces Survival in Cancer Cells. Int J Mol Sci. 2021,22(17). doi: 10.3390/ijms22179182
    Read this paper

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