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ATG14 Antibody

LS-C349213
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATG14
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ATG14 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 42kDa/55kDa, while the observed MW by Western blot was 60kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 42kDa/55kDa, while the observed MW by Western blot was 60kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID22863
  • Target name
    ATG14
  • Target description
    autophagy related 14
  • Target synonyms
    ATG14L, BARKOR, KIAA0831, beclin 1-associated autophagy-related key regulator, ATG14 autophagy related 14 homolog, Beclin 1-Interacting protein, autophagy-related protein 14-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATG14L using anti-ATG14L antibody (PB9481). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: HELA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG14L antigen affinity purified polyclonal antibody (Catalog # PB9481) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG14L at approximately 59KD. The expected band size for ATG14L is at 59KD.
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