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WB analysis of various sample lysates using GTX30072 ATG16L1 antibody. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of various sample lysates using GTX30072 ATG16L1 antibody. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of various sample lysates using GTX30072 ATG16L1 antibody. Dilution : 1:1000 Loading : 25μg per lane

ATG16L1 antibody

GTX30072
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetATG16L1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ATG16L1 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55054
  • Target name
    ATG16L1
  • Target description
    autophagy related 16 like 1
  • Target synonyms
    APG16L, ATG16A, ATG16L, IBD10, WDR30, autophagy-related protein 16-1, APG16L beta, ATG16 autophagy related 16-like 1, WD repeat domain 30
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ676U5
  • Protein Name
    Autophagy-related protein 16-1
  • Scientific Description
    The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ATG16L1 using anti-ATG16L1 antibody (A00526). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG16L1 antigen affinity purified polyclonal antibody (Catalog # A00526) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG16L1 at approximately 68 kDa. The expected band size for ATG16L1 is at 68 kDa.
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