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Western blot analysis of extracts of various cell lines using ATG16L1 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using ATG16L1 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using ATG16L1 Polyclonal Antibody at dilution of 1:1000.

ATG16L1 Polyclonal Antibody

E-AB-64109
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ATG16L1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATG16L1 using anti-ATG16L1 antibody (A00526). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG16L1 antigen affinity purified polyclonal antibody (Catalog # A00526) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG16L1 at approximately 68 kDa. The expected band size for ATG16L1 is at 68 kDa.
Product group Antibodies
Boster Bio
Anti-ATG16L1 Antibody Picoband(r)A00526-3-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetATG16L1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ATG16L1 Antibody144-60076
ApplicationsWestern Blot
ReactivityHuman
TargetATG16L1
  • SizePrice
Product group Antibodies
Biomatik
Autophagy Related Protein 16 Like Protein 1 (ATG16L1) Polyclonal AntibodyCAU21237
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Porcine, Rat
TargetATG16L1
  • SizePrice
Product group Antibodies
Bioss
ATG16L1 Recombinant AntibodyBSM-60770R
ApplicationsWestern Blot
ReactivityHuman
TargetATG16L1
  • SizePrice
Product group Antibodies
Assay Genie
ATG16L1 AntibodyCAB1871
ReactivityHuman
TargetATG16L1
  • SizePrice
Immunohistochemistry analysis in human testis and liver tissues using Anti-ATG16L1 antibody. Corresponding ATG16L1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-ATG16L1 AntibodyHPA063900
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetATG16L1
  • SizePrice
Product group Antibodies
Cusabio
ATG16L1 AntibodyCSB-PA030171
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetATG16L1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-ATG16L1 AntibodyEB07893
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetATG16L1
  • SizePrice
Product group Antibodies
MedChem Express
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TargetATG16L1
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Wild-type (WT) and ATG16L1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG16L1 antibody [N2C1], Internal (GTX110619) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
References
GeneTex
ATG16L1 antibody [N2C1], InternalGTX110619
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetATG16L1
  • SizePrice