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ATG3

ORB636089
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetATG3
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Overview

  • Supplier
    Biorbyt
  • Product Name
    ATG3 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; ELISA: 1/10000 ICC, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7A1D1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64422
  • Target name
    ATG3
  • Target description
    autophagy related 3
  • Target synonyms
    APG3, APG3-LIKE, APG3L, PC3-96, hApg3, ubiquitin-like-conjugating enzyme ATG3, APG3 autophagy 3-like, ATG3 autophagy related 3 homolog, autophagy-related protein 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9NT62
  • Protein Name
    Ubiquitin-like-conjugating enzyme ATG3
  • Scientific Description
    Mouse monoclonal antibody to ATG3
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Apg3 using anti-Apg3 antibody (A01768-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apg3 antigen affinity purified polyclonal antibody (Catalog # A01768-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apg3 at approximately 36-40 kDa. The expected band size for Apg3 is at 36 kDa.
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