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IHC-P analysis of human spleen tissue using GTX31941 ATG4A antibody. Working concentration : 5 microg/ml
IHC-P analysis of human spleen tissue using GTX31941 ATG4A antibody. Working concentration : 5 microg/ml
IHC-P analysis of human spleen tissue using GTX31941 ATG4A antibody. Working concentration : 5 microg/ml

ATG4A antibody

GTX31941
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetATG4A
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Overview

  • Supplier
    GeneTex
  • Product Name
    ATG4A antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID115201
  • Target name
    ATG4A
  • Target description
    autophagy related 4A cysteine peptidase
  • Target synonyms
    APG4 autophagy 4 homolog A; APG4A; ATG4 autophagy related 4 homolog A; AUTL2; AUT-like 2, cysteine endopeptidase; autophagin 2; autophagy-related cysteine endopeptidase 2; autophagy-related protein 4 homolog A; cysteine protease ATG4A; hAPG4A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WYN0
  • Protein Name
    Cysteine protease ATG4A
  • Scientific Description
    Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATG4A using anti-ATG4A antibody (A06539-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4A antigen affinity purified polyclonal antibody (Catalog # A06539-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG4A at approximately 50KD. The expected band size for ATG4A is at 45KD.
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