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ProductsBack/ATG4B antibody [HL2348]
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG4B antibody [HL2348] (GTX638551) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG4B antibody [HL2348] (GTX638551) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG4B antibody [HL2348] (GTX638551) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATG4B antibody [HL2348]

GTX638551
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATG4B
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Overview

  • Supplier
    GeneTex
  • Product Name
    ATG4B antibody [HL2348]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2348
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23192
  • Target name
    ATG4B
  • Target description
    autophagy related 4B cysteine peptidase
  • Target synonyms
    APG4B, AUTL1, HsAPG4B, cysteine protease ATG4B, APG4 autophagy 4 homolog B, ATG4 autophagy related 4 homolog B, AUT-like 1 cysteine endopeptidase, autophagin-1, autophagy-related cysteine endopeptidase 1, autophagy-related protein 4 homolog B, hAPG4B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4P1
  • Protein Name
    Cysteine protease ATG4B
  • Scientific Description
    Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATG4B using anti-ATG4B antibody (A02885-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human MOLT-4 whole cell lysates, Lane 8: human HEL whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: rat liver tissue lysates, Lane 11: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4B antigen affinity purified polyclonal antibody (Catalog # A02885-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG4B at approximately 48 kDa. The expected band size for ATG4B is at 48 kDa.
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