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WB analysis of various sample lysates using GTX32459 ATG7 antibody. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of various sample lysates using GTX32459 ATG7 antibody. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of various sample lysates using GTX32459 ATG7 antibody. Dilution : 1:1000 Loading : 25μg per lane

ATG7 antibody

GTX32459
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATG7
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Overview

  • Supplier
    GeneTex
  • Product Name
    ATG7 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10533
  • Target name
    ATG7
  • Target description
    autophagy related 7
  • Target synonyms
    APG7-LIKE, APG7L, GSA7, SCAR31, ubiquitin-like modifier-activating enzyme ATG7, APG7 autophagy 7-like, ATG12-activating enzyme E1 ATG7, hAGP7, ubiquitin-activating enzyme E1-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95352
  • Protein Name
    Ubiquitin-like modifier-activating enzyme ATG7
  • Scientific Description
    This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Does the Autophagy Related Gene 7 (ATG7) Have a Role in Non-Melanoma Skin Cancer? Samaka RM et al., 2020, Clin Cosmet Investig Dermatol
    Read this paper
  • Trophoblast-Specific Conditional Atg7 Knockout Mice Develop Gestational Hypertension. Aoki A et al., 2018 Nov, Am J Pathol
    Read this paper

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Figure 1. Western blot analysis of ATG7 using anti-ATG7 antibody (A00346-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG7 antigen affinity purified polyclonal antibody (Catalog # A00346-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG7 at approximately 78KD. The expected band size for ATG7 is at 78KD.
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