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ATG7 Polyclonal Antibody

RD84449A
Reddot Biotech
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetATG7
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    ATG7 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10533
  • Target name
    ATG7
  • Target description
    autophagy related 7
  • Target synonyms
    APG7 autophagy 7-like; APG7L; APG7-LIKE; ATG12-activating enzyme E1 ATG7; GSA7; hAGP7; SCAR31; ubiquitin-activating enzyme E1-like protein; ubiquitin-like modifier-activating enzyme ATG7
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATG7 using anti-ATG7 antibody (A00346-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG7 antigen affinity purified polyclonal antibody (Catalog # A00346-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG7 at approximately 78KD. The expected band size for ATG7 is at 78KD.
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