ATG9A antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATG9A stained by ATG9A antibody (GTX128427) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
![ATG9A antibody immunoprecipitates ATG9A protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of ATG9A protein by 4 μg ATG9A antibody (GTX128427) 7.5 % SDS-PAGE The immunoprecipitated ATG9A protein was detected by ATG9A antibody (GTX128427) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX128427/GTX128427_41367_IP_w_23060523_615.webp "ATG9A antibody immunoprecipitates ATG9A protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of ATG9A protein by 4 μg ATG9A antibody (GTX128427) 7.5 % SDS-PAGE The immunoprecipitated ATG9A protein was detected by ATG9A antibody (GTX128427) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
ATG9A antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATG9A stained by ATG9A antibody (GTX128427) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
ATG9A antibody
GTX128427
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATG9A
Overview
- SupplierGeneTex
- Product NameATG9A antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.98 mg/ml
- ConjugateUnconjugated
- Gene ID79065
- Target nameATG9A
- Target descriptionautophagy related 9A
- Target synonymsAPG9L1, MGD3208, mATG9, autophagy-related protein 9A, APG9 autophagy 9-like 1, APG9-like 1, ATG9 autophagy related 9 homolog A, autophagy 9-like 1 protein
- HostRabbit
- IsotypeIgG
- Protein IDQ7Z3C6
- Protein NameAutophagy-related protein 9A
- Scientific DescriptionPlays a role in autophagy.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Buono R, Tucci J, Cutri R, et al. Fasting-Mimicking Diet Inhibits Autophagy and Synergizes with Chemotherapy to Promote T-Cell-Dependent Leukemia-Free Survival. Cancers (Basel). 2023,15(24). doi: 10.3390/cancers15245870Read this paper
- Zhang X, Wang L, Ireland SC, et al. GORASP2/GRASP55 collaborates with the PtdIns3K UVRAG complex to facilitate autophagosome-lysosome fusion. Autophagy. 2019,15(10):1787-1800. doi: 10.1080/15548627.2019.1596480Read this paper
- Prabakaran T, Bodda C, Krapp C, et al. Attenuation of cGAS-STING signaling is mediated by a p62/SQSTM1-dependent autophagy pathway activated by TBK1. EMBO J. 2018,37(8). doi: 10.15252/embj.201797858Read this paper
- Merrill NM, Schipper JL, Karnes JB, et al. PI3K-C2α knockdown decreases autophagy and maturation of endocytic vesicles. PLoS One. 2017,12(9):e0184909. doi: 10.1371/journal.pone.0184909Read this paper
- Karanasios E, Walker SA, Okkenhaug H, et al. Autophagy initiation by ULK complex assembly on ER tubulovesicular regions marked by ATG9 vesicles. Nat Commun. 2016,7:12420. doi: 10.1038/ncomms12420Read this paper
- Liu Y, Takahashi Y, Desai N, et al. Bif-1 deficiency impairs lipid homeostasis and causes obesity accompanied by insulin resistance. Sci Rep. 2016,6:20453. doi: 10.1038/srep20453Read this paper
Datasheet
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