WB analysis of 40 ug of lysate from HeLa cells untreated (Lane 1), treated with 10uM Camptothecin for 4h (Lane 2) or 12h (Lane 3) using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Dilution : 1:1000
![WB analysis of gamma-irradiated HeLa cells using GTX15666 ATM (phospho Ser1981) antibody [10H11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX15666/GTX15666_1511_WB_w_23060620_125.webp "WB analysis of gamma-irradiated HeLa cells using GTX15666 ATM (phospho Ser1981) antibody [10H11].")
![ICC/IF analysis of HT-29 cells using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Left : Primary antibody Right : Negative control without primary antibody Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes Dilution : 1:50 and incubated overnight](https://www.genetex.com/upload/website/prouct_img/normal/GTX15666/GTX15666_1998_ICC-IF_w_23060620_886.webp "ICC/IF analysis of HT-29 cells using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Left : Primary antibody Right : Negative control without primary antibody Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes Dilution : 1:50 and incubated overnight")
![ICC/IF analysis of HeLa cells irradiated with UV for 4 hours using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes](https://www.genetex.com/upload/website/prouct_img/normal/GTX15666/GTX15666_278_ICC-IF_w_23060620_686.webp "ICC/IF analysis of HeLa cells irradiated with UV for 4 hours using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes")
![ICC/IF analysis of HeLa cells left untreated (left panels) or treated with 10uM Camptothecin for 4 hours (right panels) using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes](https://www.genetex.com/upload/website/prouct_img/normal/GTX15666/GTX15666_279_ICC-IF_w_23060620_514.webp "ICC/IF analysis of HeLa cells left untreated (left panels) or treated with 10uM Camptothecin for 4 hours (right panels) using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes")
![ICC/IF analysis of 293 untreated cells (left panel) or stimulated cells with 100 mJ UV (right panel) using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes](https://www.genetex.com/upload/website/prouct_img/normal/GTX15666/GTX15666_280_ICC-IF_w_23060620_691.webp "ICC/IF analysis of 293 untreated cells (left panel) or stimulated cells with 100 mJ UV (right panel) using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes")
WB analysis of 40 ug of lysate from HeLa cells untreated (Lane 1), treated with 10uM Camptothecin for 4h (Lane 2) or 12h (Lane 3) using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Dilution : 1:1000
ATM (phospho Ser1981) antibody [10H11]
GTX15666
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetATM
Overview
- SupplierGeneTex
- Product NameATM (phospho Ser1981) antibody [10H11] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:1000. ICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID10H11
- ConjugateUnconjugated
- Gene ID472
- Target nameATM
- Target descriptionATM serine/threonine kinase
- Target synonymsAT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated; ATC; ATD; ATDC; ATE; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1
- HostMouse
- IsotypeIgG1
- Protein IDQ13315
- Protein NameSerine-protein kinase ATM
- Scientific DescriptionThe protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203