
Sample (30 μg of whole cell lysate) A: HeLa B: Raji 7.5% SDS PAGE GTX112379 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ATP citrate lyase antibody [C2C3], C-term
GTX112379
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACLY
Overview
- SupplierGeneTex
- Product NameATP citrate lyase antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID47
- Target nameACLY
- Target descriptionATP citrate lyase
- Target synonymsACL, ATPCL, CLATP, ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, citrate cleavage enzyme
- HostRabbit
- IsotypeIgG
- Protein IDP53396
- Protein NameATP-citrate synthase
- Scientific DescriptionATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![ATP citrate lyase antibody [C2C3], C-term detects ATP citrate lyase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATP citrate lyase protein stained by ATP citrate lyase antibody [C2C3], C-term (GTX112379) diluted at 1:200. Blue: Hoechst 33342 staining. ATP citrate lyase antibody [C2C3], C-term detects ATP citrate lyase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATP citrate lyase protein stained by ATP citrate lyase antibody [C2C3], C-term (GTX112379) diluted at 1:200. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112379/GTX112379_40114_20160525_IFA_w_23060500_525.webp)

![ATP citrate lyase antibody [C2C3], C-term detects ACLY protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 5% SDS-PAGE ATP citrate lyase antibody [C2C3], C-term (GTX112379) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. ATP citrate lyase antibody [C2C3], C-term detects ACLY protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 5% SDS-PAGE ATP citrate lyase antibody [C2C3], C-term (GTX112379) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112379/GTX112379_40114_WB_R_w_23060500_244.webp)






