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ATP Citrate Lyase Antibody (YA829, Mouse)

HY-P80560
MedChem Express
TargetACLY
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ATP Citrate Lyase Antibody (YA829, Mouse)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID47
  • Target name
    ACLY
  • Target description
    ATP citrate lyase
  • Target synonyms
    ACL, ATPCL, CLATP, ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, citrate cleavage enzyme
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    ATP Citrate Lyase Antibody (YA829) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ATP Citrate Lyase.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACLY using anti-ACLY antibody (PB10024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MOLT4 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human T47D whole cell lysates, Lane 8: human HepG2 whole cell lysates, Lane 9: rat pancreas tissue lysates, Lane 10: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACLY antigen affinity purified polyclonal antibody (Catalog # PB10024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACLY at approximately 125 kDa. The expected band size for ACLY is at 125 kDa.
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