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ATP1B1 Polyclonal Antibody

CAC15037
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetATP1B1
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Overview

  • Supplier
    Biomatik
  • Product Name
    ATP1B1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID481
  • Target name
    ATP1B1
  • Target description
    ATPase Na+/K+ transporting subunit beta 1
  • Target synonyms
    ATP1B, sodium/potassium-transporting ATPase subunit beta-1, ATPase, Na+/K+ transporting, beta 1 polypeptide, Beta 1-subunit of Na(+),K(+)-ATPase, Na, K-ATPase beta-1 polypeptide, adenosinetriphosphatase, sodium pump subunit beta-1, sodium-potassium ATPase subunit beta 1 (non-catalytic), sodium/potassium-dependent ATPase beta-1 subunit, sodium/potassium-transporting ATPase beta-1 chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05026
  • Protein Name
    Sodium/potassium-transporting ATPase subunit beta-1
  • Scientific Description
    The ATP1B1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of ATP1B1 using anti-ATP1B1 antibody (A03469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1B1 antigen affinity purified polyclonal antibody (Catalog # A03469-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1B1 at approximately 50 kDa. The expected band size for ATP1B1 is at 50 kDa.
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