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ATP1B2 Antibody

CAB9928
Assay Genie
TargetATP1B2
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    ATP1B2 Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID482
  • Target name
    ATP1B2
  • Target description
    ATPase Na+/K+ transporting subunit beta 2
  • Target synonyms
    AMOG, sodium/potassium-transporting ATPase subunit beta-2, ATPase, Na+/K+ transporting, beta 2 polypeptide, Na, K-ATPase beta-2 polypeptide, adhesion molecule in glia, adhesion molecule on glia, sodium pump subunit beta-2, sodium-potassium ATPase subunit beta 2 (non-catalytic), sodium/potassium-dependent ATPase beta-2 subunit, sodium/potassium-dependent ATPase subunit beta-2, sodium/potassium-transporting ATPase beta-2 chain
  • Host
    Rabbit
  • Protein IDP14415
  • Protein Name
    Sodium/potassium-transporting ATPase subunit beta-2
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATP1B2 using anti-ATP1B2 antibody (A07027-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1B2 antigen affinity purified polyclonal antibody (Catalog # A07027-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1B2 at approximately 44 kDa. The expected band size for ATP1B2 is at 33 kDa.
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