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ATP2A2 / SERCA2 Antibody

LS-C815942
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetATP2A2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ATP2A2 / SERCA2 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:100 - 1:400) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID488
  • Target name
    ATP2A2
  • Target description
    ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
  • Target synonyms
    ATP2B; ATPase Ca++ transporting cardiac muscle slow twitch 2; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; calcium pump 2; cardiac Ca2+ ATPase; DAR; DD; endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA2; SR Ca(2+)-ATPase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATP2A2 using anti-ATP2A2 antibody (RP1054). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP2A2 antigen affinity purified polyclonal antibody (Catalog # RP1054) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP2A2 at approximately 115 kDa. The expected band size for ATP2A2 is at 115 kDa.
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Western blot All lanes: ATP2A2 antibody at 6microg/ml Lane 1: Mouse heart tissue Lane 2: Mouse skeletal muscle tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 115, 110, 112 kDa Observed band size: 115 kDa
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