Bio-Connect

ATP4B Polyclonal Antibody

RD77328A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetATP4B
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Reddot Biotech
  • Product Name
    ATP4B Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID496
  • Target name
    ATP4B
  • Target description
    ATPase H+/K+ transporting subunit beta
  • Target synonyms
    ATP6B; ATPase H+/K+ transporting beta subunit; ATPase, H+/K+ exchanging, beta polypeptide; ATPase, H+/K+ transporting, beta polypeptide; gastric H(+)/K(+) ATPase subunit beta; gastric H+/K+ ATPase beta subunit; gastric hydrogen-potassium ATPase, beta; potassium-transporting ATPase beta chain; potassium-transporting ATPase subunit beta; proton pump beta chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
TargetATP4B
  • SizePrice
IHC-P analysis of human gastric carcinoma tissue using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
Product group Antibodies
References
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetATP4B
  • SizePrice
Immunohistochemistry analysis in human stomach and liver tissues using HPA045400 antibody. Corresponding ATP4B RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoHistoChemistry
TargetATP4B
  • SizePrice
Product group Antibodies
Cusabio
ATP4B AntibodyCSB-PA002343EA01HU
ApplicationsELISA
TargetATP4B
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetATP4B
  • SizePrice
Mouse muscle lysates probed with ATP4B Polyclonal Antibody, Unconjugated (bs-2433R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetATP4B
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ATP4B AntibodyLS-C403857
ApplicationsELISA, ImmunoHistoChemistry
TargetATP4B
  • SizePrice
Product group Antibodies
MedChem Express
TargetATP4B
  • SizePrice
Figure 1. Western blot analysis of ATP4B using anti-ATP4B antibody (A08719-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat stomach tissue lysate, lane 2: mouse stomach tissue lysate, lane 3: SGC7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP4B antigen affinity purified polyclonal antibody (Catalog # A08719-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP4B at approximately 60KD. The expected band size for ATP4B is at 33KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetATP4B
  • SizePrice
Anti-ATP4B Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoHistoChemistry
  • SizePrice