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Formalin-fixed and paraffin embedded rat brain labeled with Anti-ATP7B Polyclonal Antibody, Unconjugated (bs-1718R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-ATP7B Polyclonal Antibody, Unconjugated (bs-1718R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-ATP7B Polyclonal Antibody, Unconjugated (bs-1718R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

ATP7B Polyclonal Antibody

BS-1718R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetATP7B
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ATP7B Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID540
  • Target name
    ATP7B
  • Target description
    ATPase copper transporting beta
  • Target synonyms
    ATPase, Cu(2+)- transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide; copper pump 2; copper-transporting ATPase 2; copper-transporting protein ATP7B; PWD; WC1; WD; Wilson disease-associated protein; WND
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35670
  • Protein Name
    Copper-transporting ATPase 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Impact of copper in Xenopus laevis liver: Histological damages and atp7b downregulation. Carotenuto R et al., 2020 Jan 30, Ecotoxicol Environ Saf
    Read more
  • miR-133a enhances the sensitivity of Hep-2 cells and vincristine-resistant Hep-2v cells to cisplatin by downregulating ATP7B expression. Wang X et al., 2016 Jun, Int J Mol Med
    Read more

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Figure 1. Western blot analysis of ATP7B using anti-ATP7B antibody (A00686-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP7B antigen affinity purified polyclonal antibody (Catalog # A00686-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP7B at approximately 157 kDa. The expected band size for ATP7B is at 157 kDa.
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