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ProductsBack/ATXN1 / SCA1 Antibody (aa686-699)

ATXN1 / SCA1 Antibody (aa686-699)

LS-C139784
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetATXN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ATXN1 / SCA1 Antibody (aa686-699)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave bands at approx 75kD and 26kD in Mouse Fetal Brain lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 84.7kD according to NP_001186234.1 and 83.8kD according to NP_033150.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).. Peptide-ELISA (1:32000) Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave bands at approx 75kD and 26kD in Mouse Fetal Brain lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 84.7kD according to NP_001186234.1 and 83.8kD according to NP_033150.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6310
  • Target name
    ATXN1
  • Target description
    ataxin 1
  • Target synonyms
    alternative ataxin1; ataxin-1; ATX1; D6S504E; SCA1; spinocerebellar ataxia type 1 protein
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ataxin 1 antigen affinity purified polyclonal antibody (Catalog # PB9422) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ataxin 1 at approximately 105 kDa. The expected band size for Ataxin 1 is at 105 kDa.
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