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Western Blot analysis of 293 cells using Ataxin-2 Polyclonal Antibody
Western Blot analysis of 293 cells using Ataxin-2 Polyclonal Antibody
Western Blot analysis of 293 cells using Ataxin-2 Polyclonal Antibody

ATXN2 Antibody

CSB-PA000944
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetATXN2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATXN2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6311
  • Target name
    ATXN2
  • Target description
    ataxin 2
  • Target synonyms
    ATX2, SCA2, TNRC13, ataxin-2, spinocerebellar ataxia type 2 protein, trinucleotide repeat-containing gene 13 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99700
  • Protein Name
    Ataxin-2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SCA2/ATXN2 using anti-SCA2/ATXN2 antibody (A01915). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCA2/ATXN2 antigen affinity purified polyclonal antibody (Catalog # A01915) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCA2/ATXN2 at approximately 140-150 kDa. The expected band size for SCA2/ATXN2 is at 140 kDa.
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