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Western Blot analysis of Hela and 293T cell using AUP1 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Hela and 293T cell using AUP1 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Hela and 293T cell using AUP1 Polyclonal Antibody at dilution of 1:500

AUP1 Polyclonal Antibody

E-AB-10796
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    AUP1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    This gene encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signalling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AUP1 using anti-AUP1 antibody (A08937-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AUP1 antigen affinity purified polyclonal antibody (Catalog # A08937-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AUP1 at approximately 42 kDa. The expected band size for AUP1 is at 42 kDa.
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