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AURKA / Aurora-A Antibody

LS-C812884
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetAURKA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AURKA / Aurora-A Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6790
  • Target name
    AURKA
  • Target description
    aurora kinase A
  • Target synonyms
    AIK, ARK1, AURA, BTAK, PPP1R47, STK15, STK6, STK7, aurora kinase A, aurora 2, aurora/IPL1-like kinase, aurora/IPL1-related kinase 1, breast tumor-amplified kinase, protein phosphatase 1, regulatory subunit 47, serine/threonine protein kinase 15, serine/threonine-protein kinase 6, serine/threonine-protein kinase aurora-A
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora A/AURKA antigen affinity purified polyclonal antibody (Catalog # A00246-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora A/AURKA at approximately 50 kDa. The expected band size for Aurora A/AURKA is at 50 kDa.
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