Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody [HL3488] (GTX641376) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody [HL3488] (GTX641376) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641376/GTX641376_T-45607_20241213_WB_Fraction_24121918_413.webp "U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody [HL3488] (GTX641376) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Autotaxin antibody [HL3488] detects secreted Autotaxin protein by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. Autotaxin stained by Autotaxin antibody [HL3488] (GTX641376) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641376/GTX641376_T-45607_20250127_IHC-P_25021923_974.webp "Autotaxin antibody [HL3488] detects secreted Autotaxin protein by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. Autotaxin stained by Autotaxin antibody [HL3488] (GTX641376) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Autotaxin antibody [HL3488] detects Autotaxin protein by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Autotaxin stained by Autotaxin antibody [HL3488] (GTX641376) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX641376/GTX641376_T-45607_20250207_ICC_IF_25022521_876.webp "Autotaxin antibody [HL3488] detects Autotaxin protein by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Autotaxin stained by Autotaxin antibody [HL3488] (GTX641376) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody [HL3488] (GTX641376) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Autotaxin antibody [HL3488]
GTX641376
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetENPP2
Overview
- SupplierGeneTex
- Product NameAutotaxin antibody [HL3488]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3488
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5168
- Target nameENPP2
- Target descriptionectonucleotide pyrophosphatase/phosphodiesterase 2
- Target synonymsATX, ATX-X, AUTOTAXIN, LysoPLD, NPP2, PD-IALPHA, PDNP2, autotaxin, E-NPP 2, autotaxin-t, ectonucleotide pyrophosphatase/phosphodiesterase family member 2, extracellular lysophospholipase D, phosphodiesterase I/nucleotide pyrophosphatase 2, plasma lysophospholipase D
- HostRabbit
- IsotypeIgG
- Protein IDQ13822
- Protein NameAutotaxin
- Scientific DescriptionThe protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5 end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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