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AVPR1A / V1a Receptor Antibody (Cytoplasmic Domain)

LS-C40158
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityRat
TargetAVPR1A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AVPR1A / V1a Receptor Antibody (Cytoplasmic Domain)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot and ELISA. Western Blot: 1:1000-1:5000 using ECL.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:50000), WB (1:1000 - 1:5000) Suitable for use in Western Blot and ELISA. Western Blot: 1:1000-1:5000 using ECL.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID552
  • Target name
    AVPR1A
  • Target description
    arginine vasopressin receptor 1A
  • Target synonyms
    AVPR V1a, AVPR1, V1aR, vasopressin V1a receptor, SCCL vasopressin subtype 1a receptor, V1-vascular vasopressin receptor AVPR1A, V1a vasopressin receptor, antidiuretic hormone receptor 1A, vascular/hepatic-type arginine vasopressin receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AVPR1A using anti-AVPR1A antibody (A03279). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AVPR1A antigen affinity purified polyclonal antibody (Catalog # A03279) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AVPR1A at approximately 60KD. The expected band size for AVPR1A is at 47KD.
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