Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Axin1 Polyclonal Antibody, Unconjugated (bs-2439R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Axin1 Polyclonal Antibody, Unconjugated (bs-2439R) at 1:200 overnight at 4°C, DAB staining.
Axin1 Polyclonal Antibody
BS-2439R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Guinea Pig, Human, Mouse, Porcine, Rat, Sheep
TargetAXIN1
Overview
- SupplierBioss
- Product NameAxin1 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID8312
- Target nameAXIN1
- Target descriptionaxin 1
- Target synonymsAXIN, CMDOH, PPP1R49, axin-1, axis inhibition protein 1, axis inhibitor 1, fused, mouse, homolog of, protein phosphatase 1, regulatory subunit 49
- HostRabbit
- IsotypeIgG
- Protein IDO15169
- Protein NameAxin-1
- ReactivityBovine, Canine, Guinea Pig, Human, Mouse, Porcine, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203
References
- Zou Q, Hou Y, Wang H, et al. Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway. EBioMedicine. 2018,31:287-298. doi: 10.1016/j.ebiom.2018.05.004Read this paper
Datasheet
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