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ProductsBack/AXL Antibody (clone 101, Preservative Free)

AXL Antibody (clone 101, Preservative Free)

LS-C818809
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetAXL
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AXL Antibody (clone 101, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flo (1:25 - 1:100) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    101
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID558
  • Target name
    AXL
  • Target description
    AXL receptor tyrosine kinase
  • Target synonyms
    ARK, AXL3, JTK11, Tyro7, UFO, tyrosine-protein kinase receptor UFO, AXL oncogene, AXL transforming sequence/gene
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AXL using anti-AXL antibody (A00226-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXL antigen affinity purified polyclonal antibody (Catalog # A00226-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AXL at approximately 140KD. The expected band size for AXL is at 98KD.
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