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AXL antibody

GTX129407
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAXL
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Overview

  • Supplier
    GeneTex
  • Product Name
    AXL antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. ELISA: 1:-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID558
  • Target name
    AXL
  • Target description
    AXL receptor tyrosine kinase
  • Target synonyms
    ARK; AXL oncogene; AXL transforming sequence/gene; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30530
  • Protein Name
    Tyrosine-protein kinase receptor UFO
  • Scientific Description
    The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae, to the liver.
    Read more
  • TGF-beta1 Promotes Zika Virus Infection in Immortalized Human First-Trimester Trophoblasts via the Smad Pathway. Trinh QD et al., 2022 Sep 27, Cells
    Read more
  • Ampelopsins A and C Induce Apoptosis and Metastasis through Downregulating AxL, TYRO3, and FYN Expressions in MDA-MB-231 Breast Cancer Cells. Huang C et al., 2019 Mar 13, J Agric Food Chem
    Read more

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Figure 1. Western blot analysis of AXL using anti-AXL antibody (A00226-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXL antigen affinity purified polyclonal antibody (Catalog # A00226-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AXL at approximately 140KD. The expected band size for AXL is at 98KD.
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