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Western blot All lanes: B3GNT2 antibody at 4microg/ml Lane 1: 293T whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47, 46 kDa Observed band size: 47 kDa
Western blot All lanes: B3GNT2 antibody at 4microg/ml Lane 1: 293T whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47, 46 kDa Observed band size: 47 kDa
Western blot All lanes: B3GNT2 antibody at 4microg/ml Lane 1: 293T whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47, 46 kDa Observed band size: 47 kDa

B3GNT2 Antibody

CSB-PA002500LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetB3GNT2
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Overview

  • Supplier
    Cusabio
  • Product Name
    B3GNT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10678
  • Target name
    B3GNT2
  • Target description
    UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
  • Target synonyms
    3-Gn-T1, 3-Gn-T2, B3GN-T2, B3GNT, B3GNT-2, B3GNT1, BETA3GNT, BGNT2, BGnT-2, beta-1, beta3Gn-T1, beta3Gn-T2, N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1, beta-1,3-N-acetylglucosaminyltransferase bGnT-1, beta-1,3-N-acetylglucosaminyltransferase bGnT-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NY97
  • Protein Name
    N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
  • Scientific Description
    Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains. Shows a marked preference for Gal(beta1-4)Glc(NAc)-based acceptors (PubMed:9892646). Probably constitutes the main polylactosamine synthase.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of B3GNT2 using anti-B3GNT2 antibody (A09524-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B3GNT2 antigen affinity purified polyclonal antibody (Catalog # A09524-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B3GNT2 at approximately 55 kDa. The expected band size for B3GNT2 is at 46 kDa.
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