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WB analysis of human pancreas lysate using GTX88963 B3GNT2 antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human pancreas lysate using GTX88963 B3GNT2 antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human pancreas lysate using GTX88963 B3GNT2 antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer

B3GNT2 antibody, Internal

GTX88963
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetB3GNT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    B3GNT2 antibody, Internal
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.1-0.3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10678
  • Target name
    B3GNT2
  • Target description
    UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
  • Target synonyms
    3-Gn-T1; 3-Gn-T2; B3GNT; B3GNT1; B3GN-T2; B3GNT-2; beta-1; beta-1,3-N-acetylglucosaminyltransferase bGnT-1; beta-1,3-N-acetylglucosaminyltransferase bGnT-2; BETA3GNT; beta3Gn-T1; beta3Gn-T2; BGNT2; BGnT-2; N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9NY97
  • Protein Name
    N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
  • Scientific Description
    This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of B3GNT2 using anti-B3GNT2 antibody (A09524-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B3GNT2 antigen affinity purified polyclonal antibody (Catalog # A09524-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B3GNT2 at approximately 55 kDa. The expected band size for B3GNT2 is at 46 kDa.
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