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BAG3 Recombinant Antibody, Biotin Conjugated

BSM-61540R-BIOTIN
Bioss Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBAG3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    BAG3 Recombinant Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Biotin
  • Gene ID9531
  • Target name
    BAG3
  • Target description
    BAG cochaperone 3
  • Target synonyms
    BAG family molecular chaperone regulator 3; BAG-3; BCL2 associated athanogene 3; BCL2-binding athanogene 3; bcl-2-binding protein Bis; Bcl-2-binding protein BIS transcript 2; BIS; CAIR-1; docking protein CAIR-1; MFM6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95817
  • Protein Name
    BAG family molecular chaperone regulator 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 2. IHC analysis of Bag3 using anti-Bag3 antibody (PB9754). Bag3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Bag3 Antibody (PB9754) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC image of CSB-PA002531LA01HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
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