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FACS analysis of mouse splenocytes using GTX51308 BAMBI antibody. Green : Primary antibody Blue : Unstained cells Dilution : 1:100
FACS analysis of mouse splenocytes using GTX51308 BAMBI antibody. Green : Primary antibody Blue : Unstained cells Dilution : 1:100
FACS analysis of mouse splenocytes using GTX51308 BAMBI antibody. Green : Primary antibody Blue : Unstained cells Dilution : 1:100

BAMBI antibody

GTX51308
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBAMBI
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Overview

  • Supplier
    GeneTex
  • Product Name
    BAMBI antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:300-1000. IHC-P: 1:50-400. FACS: 1:20-100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID25805
  • Target name
    BAMBI
  • Target description
    BMP and activin membrane bound inhibitor
  • Target synonyms
    BMP and activin membrane-bound inhibitor homolog; NMA; non-metastatic gene A protein; putative transmembrane protein NMA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13145
  • Protein Name
    BMP and activin membrane-bound inhibitor homolog
  • Scientific Description
    This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family, whose members play important roles in signal transduction in many developmental and pathological processes. The encoded protein however is a pseudoreceptor, lacking an intracellular serine/threonine kinase domain required for signaling. Similar proteins in frog, mouse and zebrafish function as negative regulators of TGF-beta, which has led to the suggestion that the encoded protein may function to limit the signaling range of the TGF-beta family during early embryogenesis. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BAMBI using anti-BAMBI antibody (A07983-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAMBI antigen affinity purified polyclonal antibody (Catalog # A07983-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAMBI at approximately 29KD. The expected band size for BAMBI is at 29KD.
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