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Western Blot analysis of HeLa cells using BARD1 Polyclonal Antibody
Western Blot analysis of HeLa cells using BARD1 Polyclonal Antibody
Western Blot analysis of HeLa cells using BARD1 Polyclonal Antibody

BARD1 Antibody

CSB-PA000974
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBARD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    BARD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID580
  • Target name
    BARD1
  • Target description
    BRCA1 associated RING domain 1
  • Target synonyms
    BRCA1-associated RING domain protein 1, BRCA1-associated RING domain gene 1, RING-type E3 ubiquitin transferase BARD1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99728
  • Protein Name
    BRCA1-associated RING domain protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BARD1 using anti-BARD1 antibody (A01646-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BARD1 antigen affinity purified polyclonal antibody (Catalog # A01646-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BARD1 at approximately 87 kDa. The expected band size for BARD1 is at 87 kDa.
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