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Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bsm-33283M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bsm-33283M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bsm-33283M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Bax Monoclonal Antibody

BSM-33283M
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBAX
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Bax Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:500-2000), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3C1
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID581
  • Target name
    BAX
  • Target description
    BCL2 associated X, apoptosis regulator
  • Target synonyms
    apoptosis regulator BAX; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; BCL2 associated X protein; BCL2-associated X protein omega; BCL2L4; bcl2-L-4; bcl-2-like protein 4
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDQ07812
  • Protein Name
    Apoptosis regulator BAX
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Kang Z et al., 2019 Dec, Toxicol In Vitro
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