Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bsm-33283M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bsm-33283M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bax Monoclonal Antibody
BSM-33283M
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetBAX
Overview
- SupplierBioss Antibodies
- Product NameBax Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- Applications SupplierWB(1:500-2000), ICC/IF(1:50-100)
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3C1
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID581
- Target nameBAX
- Target descriptionBCL2 associated X, apoptosis regulator
- Target synonymsapoptosis regulator BAX; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; BCL2 associated X protein; BCL2-associated X protein omega; BCL2L4; bcl2-L-4; bcl-2-like protein 4
- HostMouse
- IsotypeIgG
- Protein IDQ07812
- Protein NameApoptosis regulator BAX
- Storage Instruction-20°C
- UNSPSC12352203
References
- Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Kang Z et al., 2019 Dec, Toxicol In VitroRead more
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