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BAZ2A Antibody (OAAF06145)

OAAF06145
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetBAZ2A
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    BAZ2A Antibody (OAAF06145)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  • Gene ID11176
  • Target name
    BAZ2A
  • Target description
    bromodomain adjacent to zinc finger domain 2A
  • Target synonyms
    TIP5, WALp3, bromodomain adjacent to zinc finger domain protein 2A, TTF-I interacting peptide 5, TTF-I-interacting protein 5, transcription termination factor I-interacting protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • UNSPSC
    12352203

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ChIP analysis of HEK293T and NIH3T3 cells using GTX60851 TIP-5 antibody - ChIP grade. Chromatin from HEK293T and NIH3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4?C with 100 μg sheared chromatin and either 5 μl of the TIP5 antibody or 5 μl IgG which was used as negative IP control. The IP'd DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. This figure shows the recovery by the TIP5 antibody and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.
Product group Antibodies
GeneTex
TIP-5 antibody - ChIP gradeGTX60851
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