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Western Blot analysis of PC12 cells using CD239 Polyclonal Antibody.
Western Blot analysis of PC12 cells using CD239 Polyclonal Antibody.
Western Blot analysis of PC12 cells using CD239 Polyclonal Antibody.

BCAM Antibody

CSB-PA005864
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBCAM
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Overview

  • Supplier
    Cusabio
  • Product Name
    BCAM Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4059
  • Target name
    BCAM
  • Target description
    basal cell adhesion molecule (Lutheran blood group)
  • Target synonyms
    AU, B-CAM, CD239, F8/G253, LU, MSK19, basal cell adhesion molecule, Auberger b antigen, B-CAM cell surface glycoprotein, B-cell adhesion molecule, F8/G253 antigen, Lutheran blood group variant LUGA, basal cell adhesion molecule (Lu and Au blood groups)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50895
  • Protein Name
    Basal cell adhesion molecule
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD239/BCAM using anti-CD239/BCAM antibody (A03148-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD239/BCAM antigen affinity purified polyclonal antibody (Catalog # A03148-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD239/BCAM at approximately 70KD. The expected band size for CD239/BCAM is at 70KD.
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