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BCAM / CD239 Antibody

LS-C401500
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBCAM
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BCAM / CD239 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:25 - 1:100), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4059
  • Target name
    BCAM
  • Target description
    basal cell adhesion molecule (Lutheran blood group)
  • Target synonyms
    AU; Auberger b antigen; basal cell adhesion molecule; basal cell adhesion molecule (Lu and Au blood groups); B-CAM cell surface glycoprotein; B-cell adhesion molecule; CD239; F8/G253 antigen; LU; Lutheran blood group variant LUGA; MSK19
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD239/BCAM using anti-CD239/BCAM antibody (A03148-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD239/BCAM antigen affinity purified polyclonal antibody (Catalog # A03148-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD239/BCAM at approximately 70KD. The expected band size for CD239/BCAM is at 70KD.
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