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ProductsBack/BCAP / PIK3AP1 Antibody (clone OTI5D4)

BCAP / PIK3AP1 Antibody (clone OTI5D4)

LS-C172393
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetPIK3AP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BCAP / PIK3AP1 Antibody (clone OTI5D4)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:100), WB (1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5D4
  • Concentration
    0.96 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID118788
  • Target name
    PIK3AP1
  • Target description
    phosphoinositide-3-kinase adaptor protein 1
  • Target synonyms
    B cell adaptor protein; BCAP; B-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1; phosphoinositide 3-kinase adapter protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PIK3AP1 using anti-PIK3AP1 antibody (A08114). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3AP1 antigen affinity purified polyclonal antibody (Catalog # A08114) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3AP1 at approximately 90 kDa. The expected band size for PIK3AP1 is at 90,95,100 kDa.
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