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Western Blot analysis of Human placenta tissue and NIH/3T3 cell, Raji and hepG2 cell using BCAT2 Polyclonal Antibody at dilution of 1:312.5
Western Blot analysis of Human placenta tissue and NIH/3T3 cell, Raji and hepG2 cell using BCAT2 Polyclonal Antibody at dilution of 1:312.5
Western Blot analysis of Human placenta tissue and NIH/3T3 cell, Raji and hepG2 cell using BCAT2 Polyclonal Antibody at dilution of 1:312.5

BCAT2 Polyclonal Antibody

E-AB-10996
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    BCAT2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCAT2 using anti-BCAT2 antibody (A06369-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAT2 antigen affinity purified polyclonal antibody (Catalog # A06369-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAT2 at approximately 39 kDa. The expected band size for BCAT2 is at 44, 38-42 kDa.
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