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BCAT2 Polyclonal Antibody

RD75623A
Reddot Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBCAT2
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    BCAT2 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID587
  • Target name
    BCAT2
  • Target description
    branched chain amino acid transaminase 2
  • Target synonyms
    BCAM, BCATM, BCT2, HVLI, PP18, branched-chain-amino-acid aminotransferase, mitochondrial, branched chain amino-acid transaminase 2, mitochondrial, branched chain aminotransferase 2, mitochondrial, placental protein 18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15382
  • Protein Name
    Branched-chain-amino-acid aminotransferase, mitochondrial
  • Scientific Description
    This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. - This is a BCAT2 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BCAT2 using anti-BCAT2 antibody (A06369-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAT2 antigen affinity purified polyclonal antibody (Catalog # A06369-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAT2 at approximately 39 kDa. The expected band size for BCAT2 is at 44, 38-42 kDa.
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