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Bcl-XL Antibody

CAB0209
Assay Genie
TargetBCL2L1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Bcl-XL Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF,IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID598
  • Target name
    BCL2L1
  • Target description
    BCL2 like 1
  • Target synonyms
    BCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52, bcl-2-like protein 1, apoptosis regulator Bcl-X, protein phosphatase 1, regulatory subunit 52
  • Host
    Rabbit
  • Protein IDQ07817
  • Protein Name
    Bcl-2-like protein 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Bcl-XL using anti-Bcl-XL antibody (A00181). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-XL antigen affinity purified polyclonal antibody (Catalog # A00181) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl-XL at approximately 26-30 kDa. The expected band size for Bcl-XL is at 26 kDa.
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