
Wild-type (WT) and BCL10 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL10 antibody (GTX112744) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
BCL10 antibody
GTX112744
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetBCL10
Overview
- SupplierGeneTex
- Product NameBCL10 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8915
- Target nameBCL10
- Target descriptionBCL10 immune signaling adaptor
- Target synonymsCARMEN, CIPER, CLAP, IMD37, c-E10, mE10, B-cell lymphoma/leukemia 10, B cell CLL/lymphoma 10, CARD containing molecule enhancing NF-kB, CARD-containing apoptotic signaling protein, CARD-containing molecule enhancing NF-kappa-B, CARD-containing proapoptotic protein, CED-3/ICH-1 prodomain homologous E10-like regulator, cCARMEN, caspase-recruiting domain-containing protein, cellular homolog of vCARMEN, cellular-E10, hCLAP, mammalian CARD-containing adapter molecule E10
- HostRabbit
- IsotypeIgG
- Protein IDO95999
- Protein NameB-cell lymphoma/leukemia 10
- Scientific DescriptionThis gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![BCL10 antibody immunoprecipitates BCL10 protein in IP experiments. IP samples: Raji whole cell extract A. 40 μg Raji whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of BCL10 protein by 4 μg BCL10 antibody (GTX112744) 12 % SDS-PAGE The immunoprecipitated BCL10 protein was detected by BCL10 antibody (GTX112744) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent] BCL10 antibody immunoprecipitates BCL10 protein in IP experiments. IP samples: Raji whole cell extract A. 40 μg Raji whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of BCL10 protein by 4 μg BCL10 antibody (GTX112744) 12 % SDS-PAGE The immunoprecipitated BCL10 protein was detected by BCL10 antibody (GTX112744) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX112744/GTX112744_40499_IP_w_23060500_320.webp)









![ICC/IF analysis of PFA-fixed K562 cells using GTX02597 BCL10 antibody [BL10/2988R].](https://www.genetex.com/upload/website/prouct_img/normal/GTX02597/GTX02597_20210319_ICCIF_w_23053122_353.webp)
![IHC-P analysis of human tonsil tissue using GTX04716 BCL10 antibody [151]. Antigen retrieval : EDTA Buffer pH 8.0](https://www.genetex.com/upload/website/prouct_img/normal/GTX04716/GTX04716_20240325_IHC-P_24032422_706.webp)
![FACS analysis of Jurkat cells using GTX84835 BCL10 antibody [4A8]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84835/GTX84835_576_FACS_w_23061420_524.webp)
