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ProductsBack/BCL10 Mouse Monoclonal Antibody [Clone ID: OTI6A4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL10 (RC208752, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL10. Positive lysates LY418351 (100ug) and LC418351 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL10 (RC208752, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL10. Positive lysates LY418351 (100ug) and LC418351 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL10 (RC208752, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL10. Positive lysates LY418351 (100ug) and LC418351 (20ug) can be purchased separately from OriGene.

BCL10 Mouse Monoclonal Antibody [Clone ID: OTI6A4]

CF502233
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBCL10
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    BCL10 Mouse Monoclonal Antibody [Clone ID: OTI6A4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI6A4
  • Gene ID8915
  • Target name
    BCL10
  • Target description
    BCL10 immune signaling adaptor
  • Target synonyms
    CARMEN, CIPER, CLAP, IMD37, c-E10, mE10, B-cell lymphoma/leukemia 10, B cell CLL/lymphoma 10, CARD containing molecule enhancing NF-kB, CARD-containing apoptotic signaling protein, CARD-containing molecule enhancing NF-kappa-B, CARD-containing proapoptotic protein, CED-3/ICH-1 prodomain homologous E10-like regulator, cCARMEN, caspase-recruiting domain-containing protein, cellular homolog of vCARMEN, cellular-E10, hCLAP, mammalian CARD-containing adapter molecule E10
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO95999
  • Protein Name
    B-cell lymphoma/leukemia 10
  • Scientific Description
    Carrier-free (BSA/glycerol-free) BCL10 mouse monoclonal antibody, clone OTI6A4 (formerly 6A4)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Bcl10 using anti-Bcl10 antibody (PB9525). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl10 antigen affinity purified polyclonal antibody (Catalog # PB9525) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl10 at approximately 28 kDa. The expected band size for Bcl10 is at 26 kDa.
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