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ProductsBack/BCL2L11 Antibody (Phospho-Ser59) (OASG00769)

BCL2L11 Antibody (Phospho-Ser59) (OASG00769)

OASG00769
Aviva Systems Biology
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetBcl2l11
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    BCL2L11 Antibody (Phospho-Ser59) (OASG00769)
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID12125
  • Target name
    Bcl2l11
  • Target description
    BCL2 like 11
  • Target synonyms
    1500006F24Rik, Bim, Bod, bcl2-L-11, bcl-2-like protein 11, BCL2-like 11 (apoptosis facilitator), Bcl2 interacting mediator of cell death, bcl2-interacting mediator of cell death
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Bim/Bcl2l11 using anti-Bim/Bcl2l11 antibody (A01552-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/Bcl2l11 antigen affinity purified polyclonal antibody (Catalog # A01552-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/Bcl2l11 at approximately 22 kDa. The expected band size for Bim/Bcl2l11 is at 22 kDa.
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