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ProductsBack/BCMA antibody [Vicky-1]

BCMA antibody [Vicky-1]

GTX17323
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetTNFRSF17
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Overview

  • Supplier
    GeneTex
  • Product Name
    BCMA antibody [Vicky-1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 5 microg/mL. FACS: 1 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Vicky-1
  • Conjugate
    Unconjugated
  • Gene ID608
  • Target name
    TNFRSF17
  • Target description
    TNF receptor superfamily member 17
  • Target synonyms
    B cell maturation antigen; B-cell maturation factor; B-cell maturation protein; BCM; BCMA; CD269; TNFRSF13A; tumor necrosis factor receptor superfamily member 17
  • Host
    Rat
  • Isotype
    IgG1
  • Protein IDQ02223
  • Protein Name
    Tumor necrosis factor receptor superfamily member 17
  • Scientific Description
    BCMA is involved in B-cell terminal maturation. The open reading frame of the gene encoding BCMA (BCM) predicts a 184-amino acid protein with a single transmembrane domain that has no homology with any known protein sequences.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCMA using anti-BCMA antibody (A01014-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCMA antigen affinity purified polyclonal antibody (Catalog # A01014-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCMA at approximately 20KD. The expected band size for BCMA is at 20KD.
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