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BDH1 Antibody, HRP conjugated

CSB-PA002648LB01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetBDH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    BDH1 Antibody, HRP conjugated
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Gene ID622
  • Target name
    BDH1
  • Target description
    3-hydroxybutyrate dehydrogenase 1
  • Target synonyms
    BDH, SDR9C1, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, (R)-3-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase (heart, mitochondrial), 3-hydroxybutyrate dehydrogenase, type 1, epididymis secretory sperm binding protein, short chain dehydrogenase/reductase family 9C member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02338
  • Protein Name
    D-beta-hydroxybutyrate dehydrogenase, mitochondrial
  • Scientific Description
    D-beta-Hydroxybutyrate dehydrogenase mitochondria has been purified to apparent homogeneity. The membrane-bound enzyme is first released by phospholipase A digestion of the mitochondria.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BDH1 using anti-BDH1 antibody (A09277-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: human COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDH1 antigen affinity purified polyclonal antibody (Catalog # A09277-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BDH1 at approximately 31KD. The expected band size for BDH1 is at 38KD.
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