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BDH1 Antibody (YA4167, Mouse)

HY-P84470
MedChem Express
TargetBDH1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    BDH1 Antibody (YA4167, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID622
  • Target name
    BDH1
  • Target description
    3-hydroxybutyrate dehydrogenase 1
  • Target synonyms
    (R)-3-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase (heart, mitochondrial); 3-hydroxybutyrate dehydrogenase, type 1; BDH; D-beta-hydroxybutyrate dehydrogenase, mitochondrial; epididymis secretory sperm binding protein; SDR9C1; short chain dehydrogenase/reductase family 9C member 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    BDH1 Antibody (YA4167) is a mouse-derived and non-conjugated IgG1 antibody, targeting to BDH1. It can be applicated for WB, IHC-P, ELISA assays, in the background of human, mouse.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BDH1 using anti-BDH1 antibody (A09277-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: human COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDH1 antigen affinity purified polyclonal antibody (Catalog # A09277-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BDH1 at approximately 31KD. The expected band size for BDH1 is at 38KD.
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