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U251 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37C for 20 min; Antibody incubation with BDNF Polyclonal Antibody, Unconjugated (bs-4989R) 1:200, 90 minutes at 37C; followed by a conjugated secondary antibody (bs-0295G-FITC) at 37C for 90 minutes, DAPI (5ug/ml) was used to stain the cell nuclei.
U251 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37C for 20 min; Antibody incubation with BDNF Polyclonal Antibody, Unconjugated (bs-4989R) 1:200, 90 minutes at 37C; followed by a conjugated secondary antibody (bs-0295G-FITC) at 37C for 90 minutes, DAPI (5ug/ml) was used to stain the cell nuclei.
U251 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37C for 20 min; Antibody incubation with BDNF Polyclonal Antibody, Unconjugated (bs-4989R) 1:200, 90 minutes at 37C; followed by a conjugated secondary antibody (bs-0295G-FITC) at 37C for 90 minutes, DAPI (5ug/ml) was used to stain the cell nuclei.

BDNF Polyclonal Antibody

BS-4989R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBDNF
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    BDNF Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID627
  • Target name
    BDNF
  • Target description
    brain derived neurotrophic factor
  • Target synonyms
    abrineurin; ANON2; brain-derived neurotrophic factor; BULN2; neurotrophin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23560
  • Protein Name
    Brain-derived neurotrophic factor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Young rats with increased circulatory asymmetric dimethylarginine exhibited spatial deficit and alterations in dorsal hippocampus brain-derived neurotrophic factor and asymmetric dimethylarginine: Effects of melatonin. Sheen JM et al., 2019 Nov, Int J Dev Neurosci
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  • Sitagliptin enhances the neuroprotective effect of pregabalin against pentylenetetrazole-induced acute epileptogenesis in mice: Implication of oxidative, inflammatory, apoptotic and autophagy pathways. Nader MA et al., 2018 May, Neurochem Int
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  • Non-viral liposome-mediated transfer of brain-derived neurotrophic factor across the blood-brain barrier. Xing Y et al., 2016 Apr, Neural Regen Res
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  • Neurotrophic Activity of Cultured Cell Line U87 is Up-Regulated by Proline-Rich Polypeptide Complex and Its Constituent Nonapeptide. Zablocka A et al., 2015 Oct, Cell Mol Neurobiol
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