U251 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37C for 20 min; Antibody incubation with BDNF Polyclonal Antibody, Unconjugated (bs-4989R) 1:200, 90 minutes at 37C; followed by a conjugated secondary antibody (bs-0295G-FITC) at 37C for 90 minutes, DAPI (5ug/ml) was used to stain the cell nuclei.
U251 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37C for 20 min; Antibody incubation with BDNF Polyclonal Antibody, Unconjugated (bs-4989R) 1:200, 90 minutes at 37C; followed by a conjugated secondary antibody (bs-0295G-FITC) at 37C for 90 minutes, DAPI (5ug/ml) was used to stain the cell nuclei.
BDNF Polyclonal Antibody
BS-4989R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBDNF
Overview
- SupplierBioss
- Product NameBDNF Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID627
- Target nameBDNF
- Target descriptionbrain derived neurotrophic factor
- Target synonymsANON2, BULN2, neurotrophic factor BDNF precursor form, abrineurin, neurotrophin, proBDNF
- HostRabbit
- IsotypeIgG
- Protein IDP23560
- Protein NameNeurotrophic factor BDNF precursor form
- Storage Instruction-20°C
- UNSPSC12352203
References
- Sheen JM, Yu HR, Tain YL, et al. Young rats with increased circulatory asymmetric dimethylarginine exhibited spatial deficit and alterations in dorsal hippocampus brain-derived neurotrophic factor and asymmetric dimethylarginine: Effects of melatonin. Int J Dev Neurosci. 2019,78:83-89. doi: 10.1016/j.ijdevneu.2019.09.003Read this paper
- Nader MA, Ateyya H, El-Shafey M, et al. Sitagliptin enhances the neuroprotective effect of pregabalin against pentylenetetrazole-induced acute epileptogenesis in mice: Implication of oxidative, inflammatory, apoptotic and autophagy pathways. Neurochem Int. 2018,115:11-23. doi: 10.1016/j.neuint.2017.10.006Read this paper
- Xing Y, Wen CY, Li ST, et al. Non-viral liposome-mediated transfer of brain-derived neurotrophic factor across the blood-brain barrier. Neural Regen Res. 2016,11(4):617-22. doi: 10.4103/1673-5374.180747Read this paper
- Zabłocka A, Mitkiewicz M, Macała J, et al. Neurotrophic Activity of Cultured Cell Line U87 is Up-Regulated by Proline-Rich Polypeptide Complex and Its Constituent Nonapeptide. Cell Mol Neurobiol. 2015,35(7):977-86. doi: 10.1007/s10571-015-0192-8Read this paper
Datasheet
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