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ProductsBack/beta-Gal antibody [N2C3]

beta-Gal antibody [N2C3]

GTX104360
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGLB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    beta-Gal antibody [N2C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.35 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2720
  • Target name
    GLB1
  • Target description
    galactosidase beta 1
  • Target synonyms
    EBP, ELNR1, MPS4B, beta-galactosidase, acid beta-galactosidase, elastin binding protein, elastin receptor 1, 67kDa, lactase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16278
  • Protein Name
    Beta-galactosidase
  • Scientific Description
    This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the terminal beta-galactose from ganglioside substrates and other glycoconjugates. Defects in this gene are the cause of GM1-gangliosidosis and Morquio B syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Chang YC, Su CY, Chen MH, et al. Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis. Mol Cancer. 2017,16(1):135. doi: 10.1186/s12943-017-0687-7
    Read this paper
  • Nagai-Okatani C, Minamino N. Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure. PLoS One. 2016,11(6):e0150210. doi: 10.1371/journal.pone.0150210
    Read this paper

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Figure 1. Western blot analysis of GLB1/Beta-galactosidase using anti-GLB1/Beta-galactosidase antibody (A01829-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLB1/Beta-galactosidase antigen affinity purified polyclonal antibody (Catalog # A01829-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLB1/Beta-galactosidase at approximately 65-85 kDa. The expected band size for GLB1/Beta-galactosidase is at 73 kDa.
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