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ProductsBack/beta-Gal antibody [OTI5H2]
FACS analysis of HEK293T cells with/without human GLB1 transfection using GTX04880 beta-Gal antibody [OTI5H2]. Red : HEK293T cells with human GLB1 transfection Blue line : HEK293T cells without human GLB1 transfection
FACS analysis of HEK293T cells with/without human GLB1 transfection using GTX04880 beta-Gal antibody [OTI5H2]. Red : HEK293T cells with human GLB1 transfection Blue line : HEK293T cells without human GLB1 transfection
FACS analysis of HEK293T cells with/without human GLB1 transfection using GTX04880 beta-Gal antibody [OTI5H2]. Red : HEK293T cells with human GLB1 transfection Blue line : HEK293T cells without human GLB1 transfection

beta-Gal antibody [OTI5H2]

GTX04880
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetGLB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    beta-Gal antibody [OTI5H2]
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5H2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2720
  • Target name
    GLB1
  • Target description
    galactosidase beta 1
  • Target synonyms
    EBP, ELNR1, MPS4B, beta-galactosidase, acid beta-galactosidase, elastin binding protein, elastin receptor 1, 67kDa, lactase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16278
  • Protein Name
    Beta-galactosidase
  • Scientific Description
    This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome. [provided by RefSeq, Nov 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GLB1/Beta-galactosidase using anti-GLB1/Beta-galactosidase antibody (A01829-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLB1/Beta-galactosidase antigen affinity purified polyclonal antibody (Catalog # A01829-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLB1/Beta-galactosidase at approximately 65-85 kDa. The expected band size for GLB1/Beta-galactosidase is at 73 kDa.
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